Effects of FAK^I936/I998 expression on FA dynamics.

<p>(A) Representative TIRF images of FAK, FAK^I936/I998 and FAK^-/- cells. Formation of nascent adhesions at the front and dissociation of FAs at the rear (arrows) in FAK, FAK^I936/I998 and FAK^-/- cells transfected with CFP-paxillin. Arrows point to disassembling FAs at the trailing edge. Insets are magnified views of nascent adhesions over time in the yellow ellipses. Images were acquired at 1 min intervals for 1 h (scale bar, 5 μm) and representative images from the time-lapse sequence are shown at 2 min intervals. Colour images represent the superposition of images taken at t₀ (red) and t₆₀ (green). (B) Percentages of stable FAs were calculated from images in (A) as the number of FAs in FAK, FAK^I936/I998 and FAK^-/- cells at t = 60 min compared to t = 0. (P = 0.0055, F = 9.145; *p<0.03, n = 3 independent experiments).