DENV-2 NS3 with Cav-1 colocalize at early stages after infection.

<p>(A) HMEC-1cells were infected with DENV-2 and double stained at different times after infection (12, 24 and 48hpi) or were mock infected (not shown). In all cases, cells were double stained with mouse monoclonal antibodies against NS3 (green) and the lipid raft marker Cav-1 (red). Nuclear DNA was stained with DAPI. (B) Slides were analyzed by confocal laser scanning microscopy (Leica SP5 Objective HCXPLAPO63 63Oil). The graph shows the colocalization index. (C) HMEC-1 cells were transfected with (a) NS2BNS3pro-GFP, (b) NS3pro-GFP, and,(c) NS2B-GFP. After 24 h, they were stained with the lipid raft marker Cav-1 (red), and colocalization was analyzed.