Cell death in AR42J cells after treatment was measured by flow cytometry.

<p>(A), Flow cytometric analysis of AR42J cells stained with annexin V-APC and 7-AAD at 24 h, apoptotic cells are presented in the right-lower quadrant of the figure (Q4), necrotic cells in the right-upper quadrant (Q2), living cells in the left-lower quadrant (Q3), and cell debris in the left-upper quadrant(Q1). (B), Analytical data showing the apoptotic indices and necrotic indices in both LV-control and LV-shGRP78 treated cells. Results are expressed as means ± SE. Con: treated by PBS as control; Cer: treated by cerulein; Cer+LPS: treated by cerulein plus LPS. <i>* p</i><0.05, vs. the control group; <i># p</i><0.05, vs. LV-control cells after the same treatment. Data shown represent at least three independent experiments.