Degradation of MAVS in RV infected HT29 cells and NSP1 transfected HEK293 cells.

<p><b>A, B, C and D)</b> HT29 cells were infected with the indicated strains of RV (1 M.O.I.) and harvested at various time points. Total cellular protein was harvested and separated by SDS-PAGE. Immunoblot analyses were performed with antibodies to the cellular proteins MAVS, IRF3, GAPDH and the viral proteins NSP1 and NSP3. Porcine OSU, bovine A5-13, A5-16 and human Wa were harvested at 2, 4, 8 and 12 hpi. Murine EW, rhesus RRV, human KU and DS-1 were lysed at 6 and 12hpi. Band intensities of MAVS and IRF3 were normalized to the loading control GAPDH and were expressed as a percentage of the protein in mock infected cells. The data represent the means ± SD of three independent experiments. <b>E)</b> Effects of NSP1 proteins of different RV strains on human MAVS. HEK293 cells were transfected with vectors encoding indicated RV NSP1 proteins and harvested at 24 h p.i. Proteins in cell lysates were resolved by SDS-PAGE and analyzed by immunoblotting for MAVS, IRF3 and Anti-His antibody.