Impact of cinnamon extract supplementation on insulin sensitivity in the brain and the influence on cortical and locomotor activity in B6.V-<i>Lep</i>^ob/J (ob/ob) mice.

<p>A: Representative Western Blot out of 3 independent experiments of tyrosine phosphorylation (PY) of IR and AKT (S473) and protein expression of IR after intravenous insulin injection in overnight-fasted cinnamon extract-supplemented (Cinn.) ob/ob mice (<i>n</i> = 2–5 replications per condition). B: Analysis of basal power spectral density (indicated for delta (0.5–4 Hz), theta (4–8 Hz), alpha (8–12 Hz), and beta (12–30 Hz) frequencies) using fast Fourier transformation and estimated by ECoG in ob/ob mice supplemented with cinnamon extract (cross-hatched) or vehicle solution (filled). Basal ECoG measurements were conducted during a 4-day period (<i>n</i> = 7–8/group; **<i>P</i><0.005 between cinnamon or vehicle treatment. C: Averaged locomotor activity after cinnamon (cross-hatched) or vehicle (filled) supplementation during the day (7 AM to 7 PM) and night (7 PM to 7 AM) cycle from a 4-d period (***<i>P</i><0.001). D: Effect of acute intracerebroventricular (i.c.v.) insulin application (indicated as % of i.c.v. injected NaCl solution) on activity in cinnamon extract (cross-hatched) treated ob/ob mice compared to vehicle treatment (filled). *<i>P</i><0.05 to vehicle supplementation. Data are presented as mean ± SEM. Ins, insulin.