Ultrastructural morphologies of fungal melanin pigments isolated from mycelium and secreted to culture media.

<p><b>A–B</b> are SEM images of synthetic melanin obtained by tyrosine oxidation with H₂O₂ (Sigma M8136); <b>C–D</b> SEM images of Melanin extracted from mycelium, this pigment displays spherical granules tightly aggregated with an average size diameter of 100–300 nm; <b>E–F</b> Melanin extracted from culture media display amorphous material without definable structure composed by tightly packed of various sizes. <i>M. fijiensis</i> pigments were obtained using NaOH extraction, acid precipitation and solvent purification methods. The scale bars are showed in each image.