Expression of NIS mRNA and protein in baculovirus-infected Hela cells.

<p>(A) Total RNA samples from baculovirus-infected Hela and MRC5 cells were extracted. NIS mRNA expression normalized to the GAPDH endogenous reference was detected by qRT-PCR. Results are expressed as means ± SD of three independent experiments. (B) Total cell lysates were prepared from Hela and MRC5 cells infected with recombinant baculovirus as described in Materials and Methods. NIS protein (∼97 kDa) expression was analyzed by Western blot, and GAPDH (∼36 kDa) was used as an internal control.