Solution structure of ChxR_Eff.

<p><i>A,</i> Cartoon ribbon diagram of the minimized mean structure of <i>C. trachomatis</i> ChxR_Eff (residues 114–229) colored blue (N-terminus) to red (C-terminus). The topology of ChxR_Eff is β6–β7–β8–β9–α4–β10–α5–α6–α7–β11–β12. <i>B,</i> Surface representation of electrostatic potential, generated by DelPhi <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0091760#pone.0091760-Rocchia1" target="_blank">[40]</a>, of ChxR_Eff. Color scheme represents regions of negative (<i>red</i>) and positive (<i>blue</i>) charge density contoured at ±3 <i>e</i>/<i>kT</i>. Structure is oriented the same as panel A. <i>C,</i> Surface representation of ChxR_Eff with surface exposed side chains targeted for site directed mutagenesis colored magenta. Structure is oriented the same as panel A. All three panels are rotated 180° on the <i>right</i>.