Distribution of pherotype-characterized strains according to the ability to form <i>in vitro</i> biofilms.

<p>A total of 90 strains with pherotype CSP1 (n = 67) or CSP2 (n = 23) were screened for their ability to form biofilms in 96-well plates without the addition of synthetic CSP. After 24h of incubation, the resulting biofilm was measured by crystal violet staining. Each plotted value is an average of 9 replicates normalized by the average biofilm mass measured for TIGR4 strain (OD_595nm = 0.147). Horizontal lines represent the relative biofilm formation of R36A, TIGR4 and their isogenic switched-pherotype mutant strains.