Myogenesis is associated with increased <i>MyoG</i> expression.

<p><b>A</b>) MSCs proliferation and differentiation in DMEM/10% FBS media. Myotube formation was observed from day 12, reaching a maximum at day 16. <b>B</b>) <i>MyoG</i> expression from RNA extracted and analyzed by real time RT-PCR. <i>MyoG</i> expression gradually increased with time. <b>C</b>) MyoG protein expression at Day 10 and Day 16 was determined by Western blot analysis and the protein intensity was measured by using Image J program. MyoG protein in Day 16 cells was significantly higher than in Day 10 cells. <b>D</b>) Immunocytochemistry analysis of Day 10 and Day 16 cells stained with MyoG antibody. Day 16 showed higher MyoG nuclear expression as compared with Day 10 proliferating cells. <b>E)& F)</b><i>MyoG</i> mRNA and protein expression of MSCs transfected with MyoG shRNA. <i>MyoG</i> expression was decreased in both RNA and protein level in MyoG_kd cells relative to MyoG_wd cells. <b>G</b>) Immunostaining of MyoG_wd or MyoG_kd cells with MyoG antibody. A significant decrease in nuclear MyoG protein expression was observed in MyoG_kd cells. <b>H</b>) Giemsa staining performed in MyoG_wd and MyoG_kd cells. Myotube formation was decreased in MyoG_kd cells relative to MyoG_wd cells. Day 10 and MyoG_wd represents control, respectively (mean ±S.D., n = 3). <i>p</i>-value indicates the statistical significance of the data and different letters (a and b) in graph show significant differences among groups.