Determining optimal agonist concentrations on hCMEC/D3 and monocytes.

<p>Endothelial cells and monocytes were stimulated overnight with varying doses of TNF and LPS, respectively. Induced eMP were measured by flow cytometry using PE-anti-CD105 mAb (A). MP released from monocytes were counted directly from the cell suspension prior to any purification process using labelling with FITC-anti-CD31 mAb (B). Post purification, MP from non-stimulated (NS) and LPS-stimulated monocytes were enumerated and the supernatant (SN) from the final centrifugation process was also checked to ensure clearance of mMP (C). Experiments were performed at least 3 times in triplicates. Data are expressed as mean ± SD. *<i>p</i><0.05, **<i>p</i><0.01 ***<i>p</i><0.001.