Apigenin induces the apoptosis of ANA-1 cells.

<p>A. The quantification of flow cytometry analyses. The flow cytometry data showed one representative sub-G₁ result. B. The apoptotic rates are given as the percentage of cells with sub-G₁ DNA content. The values are given as the mean ± SD. *, <i>p</i><0.05, versus the control. C. ANA-1 macrophages were exposed to different concentrations of apigenin for 24 h and 48 h. The flow cytometry data showed one representative dual staining result. D. Early- (%) and late-stage (%) apoptotic cell death were detected by staining cells with Annexin V-FITC and PI and analyzing by flow cytometry. The values are given as the mean ± SD. *, <i>p</i><0.05, **, <i>p</i><0.01, versus the control. E. the ANA-1 cells were exposed to apigenin for 48 h. The images of TUNEL positive cells were captured by flow cytometry and a confocal microscopy (×200).