Quantification of self-ligation efficiency in iPCR with different DNA dilutions and different reagents.

<p><b>A</b>. Effect of DNA dilution during ligation on iPCR amplification of staggered-end fragments from HsInv0340 of 32 kb (AB) and 40 kb (BD) and the A–D concatamer. <b>B</b>. Effect of different reagent concentrations during ligation on iPCR amplification of blunt-end fragments from HsInv0286 of 33 kb (BD) and 41 kb (AB). The A–D fragment with 10 ng/µl of DNA and the 41 kb fragment with no additional reagent were selected as reference to calculate the relative amplification in <b>A</b> and <b>B</b>, respectively. NA18517 DNA that is heterozygous for both inversions was used in the analysis.