<i>acs-3(ft5)</i> and <i>nhr-25(ku217)</i> mutants are sensitive to distinct, but overlapping stresses.

<p>Sensitivity of L4 larvae of the indicated strains to osmotic stress (A), 35°C thermal stress (B), and oxidative stress (C). For osmotic stress assays (A), animals were scored for viability 24 hours after being placed on plates containing the indicated concentration of salt (50–500 mM NaCl). For thermal stress assays (B), animals were scored for viability every three hours from 0–12 hours. For oxidative stress assays, animals were either placed in 250 μM juglone or an equal amount of ethanol vehicle control for 20 minutes. The animals were then washed onto OP50 plates, allowed to recover for 18 hours, and then scored for viability. Asterisks indicate a statistically significant difference in survival relative to WT controls (two tailed T-test, p<0.05). Detailed statistical analyses, including all pair-wise comparisons are presented in Figure S7.