ARV and NBV p10 proteins form homo- but not heteromultimers.

<p>(A) Representative images of sensitized emission FRET, showing the donor and acceptor channels, and the calculated normalized FRET (NFRET) image. Control images were obtained from cells expressing EGFP directly linked to mCherry (GFP-mCh) or ARV p10-EGFP cotransfected with free mCherry (ARV-GFP+mCh) as negative FRET controls, or from cells co-expressing p14 FAST proteins linked to EGFP and mCherry as positive FRET controls for a known, multimeric membrane protein. Images acquired from cells co-expressing EGFP- and mCherry-tagged ARV p10 constructs (ARV-GFP+ARV-mCh) were used to detect ARV p10 multimerization. NFRET range is denoted by color gradations. Scale bars = 10 µm. (B) Fitted Gaussian distributions of twenty calculated NFRET images from two separate experiments were used to calculate the mNFRET (top) and mean pixel amplitude (bottom) from cells transfected or co-transfected with the indicated fluorescent probes. Boxes indicate mNFRET standard deviations, + denotes mean mNFRET, lines are the median mNFRET, and whiskers indicate min and max mNFRETs. Error bars in pixel amplitude panel represent standard error propagated within and across experiments.