PLP deficiency causes CABs in HeLa cells.

<p>(A) Western blots showing that RNAi against <i>PDXK</i> in HeLa cells reduces the level of the PDXK protein to the 38% of the control level. (B–D) Examples of control and <i>PDXK</i> RNAi HeLa cells exposed to different glucose concentrations; the metaphase in panel D exhibits extensive chromosome breakage (ECB); the thin arrows point to chromosome fragments and the thick arrow to a dicentric chromosome. Scale bar, 10 µm. (E) CAB frequencies in mock-treated and <i>PDXK</i> RNAi (abbreviated with RNAi) cells incubated in media containing different glucose (G) or fructose (F) concentrations with or without PLP (2 mM); §, includes cells with ECB; * and †, significantly different in χ² test with p<0.05 and p<0.001 respectively. (F) 4-DP (30 mM) induces CABs in HeLa cells, and this effect is potentiated by G; in contrast, ALA (10 mM) reduces the frequency of CABs induced by RNAi against <i>PDXK</i>. §, includes cells with ECB; * and †, significantly different in χ² test with p<0.001 and p<0.01, respectively.