Tobacco dependence treatment medications (varenicline, bupropion) alter the larval zebrafish nicotine response.

<p>(<b>A–C</b>) Larvae pretreated in varenicline (50 μM) overnight and challenged with stimulus at 6 dpf (± SE). Varenicline attenuates (<b>A</b>) 20 μM nicotine response, but not (<b>B</b>) 50 μM cinnamon oil or (<b>C</b>) 25 μM mustard oil response. (<b>D</b>) Acute treatment with 50 μM varenicline does not affect locomotion at 5 dpf (± SE). (<b>E–F</b>) Mean cumulative distance traveled in the 4 minutes post stimulus exposure as a percent of the average untreated stimulus response (± SE). Wash Nic = 24-hour washout period following acute nicotine experiment and re-tested at 7 dpf. Acute early and acute late response represents the first 4 minutes and last 4 minutes, respectively, post drug exposure at 5 dpf. (<b>E</b>) Movement quantitation for varenicline experiments. (<b>F</b>) Movement quantitation for bupropion experiments. n≥30 larvae per condition; * = p<0.05; Students t-test.