Involvement of HRK in AT2R-mediated apoptosis in PC-3 prostate cancer cells.

<p><b>A,</b> HRK mRNA expression in PC3 cells transduced with the indicated doses. <b>B and C</b>, PC3 cells were treated with 20 nmol/L HRK siRNA or control siRNA and Ad-G-AT2R-EGFP (200 ifu/cell) as described in Materials and Methods followed 48 h later by real-time RT-PCR analysis (<b>B</b>) of either HRK mRNA or assessment of cells with apoptotic-like morphology(<b>C</b>). Columns, mean from three separate experiments in each case; bars, SE. *, P<0.05 versus control cells.