Chk2 is activated in response to Gltn.

<p>(<b>A</b>) Western blotting of Check point kinases in response to Gltn. UV light serves as a positive control for Chk1 and Chk2 phospohorylation. Results are representative of triplicate experiments. (<b>B</b>) MDA-MB-468 cells were treated with Gltn for 72 hours prior to BrdU labeling. BrdU was quantified using FACs and results are representative of three independent experiments. (<b>C</b>) Immunofluorescent images of γH2A.X in Gltn treated cells. Cells were treated with Gltn daily for the indicated time periods and probed with an anti-Ser-129-H2A.X antibody for two hours. Doxorubicin treatment serves as a positive control for DNA damage. Results are representative of three independent experiments.