Calcineurin signaling restores Treg proliferation in the presence of Rapa.

<p>CFSE-labeled FACS-sorted Tregs were co-cultured with B6-derived DCs and IL-2 with or without Rapa or CsA in the absence (A) or presence (B) of anti-CD3 antibody. One representative histogram showing Treg CFSE dilution in each condition is shown. (C) The division index of Tregs cultured in the presence of anti-CD3 antibody was normalized to the control and is plotted as mean ± SEM of <i>n</i> = 3 independent experiments. <i>*p</i><0.01 by unpaired, two-tailed Student t test.