NDK-1 functions in DTCs.

<p>NDK-1::GFP shows expression in distal tip cells (DTCs) in TTV2 (<b>B, D</b>) and TTV3 (<b>F</b>) translational reporter lines in L3 (<b>A, B</b>), L4 (<b>C, D</b>) larvae and adults (<b>E, F and I</b>). <b>A</b>, <b>C</b>, and <b>E</b> are the corresponding DIC images of <b>B</b>, <b>D</b>, and <b>F</b>, respectively. DTC locations are indicated with arrows. We note that on panel <b>D</b> GFP expression in DTC (marked by an arrow) is overshadowed by intense expression of autofluorescent granules in the intestine. <b>E, F and I</b>: gonads were isolated from adult animals. <b>H</b>: DIC image of an adult wild-type (N2) gonad arm. <b>G</b>: Variation and distribution of DTC migration phenotypes observed in <i>ndk-1</i>(<i>ok314</i>) mutants (see details in the main text). The migratory path of DTCs is marked by dashed lines. <b>I</b>: In TTV3 lines carrying the integrated transgene, NDK-1::GFP expression is also observed in gonadal sheath cells. DIC, fluorescent and merged images derive from the gonadal loop region of a transgenic animal. On the DIC panel white arrows show dying cells, which are surrounded by sheath cells strongly expressing NDK-1::GFP (white arrows on the fluorescent and merged panels).