Understanding the Role of LIM domain-containing protein 1 in Oral Carcinoma

<p>The aim of our study was to analyze the alterations in a candidate tumor suppressor gene (TSGs) LIM domains-containing protein 1 (LIMD1) which is encoded at chromosome 3p21.3, a region commonly deleted in many solid malignancies. However, the function of LIMD1 is unknown. In the current study, we analyze 38, tumor and adjacent normal samples for mutational screening and 46 samples for Immunohistochemistry expression analysis for LIMD1 protein. We found a synonymous, heterozygous mutation on NCBI ref SNPs viz. rs267236 and rs267237 on exon – 1. Whereas it was also observed that with increased in tumor grade the expression of LIMD1 protein got down regulated. LIMD1 Overall percentage alteration ragging from 50% in mild dysplastic lesion to 77% in stage III+IV carcinoma. With the mutation observed we can indicate some co-relation of these SNPs with the development of Oral Cancer. As it may have some role in the pre-mRNA formation which later affect the translational activities and LIMD1 protein formation, which leads to the down regulation of the protein in tumor samples. Down regulation of the protein in carcinoma samples suggest its role as tumor suppressor gene, and loss of which promote carcinogenesis.