Condensin localization decreases on acrocentric short arms in the presence of dnTRF2.

<p>Combined immunostaining-FISH for (A) SMC4 (green) and rDNA (red) on metaphase chromosomes from control and 36 hour dnTRF2-expressing cells. (B) The amount of SMC4 was quantitated by measuring arbitrary fluorescence along the length of the chromosomes and plotting signal intensity as a line plot. A line begins at the p arm (0 on x-axis) and extends to the telomere of the q arm (∼150+ on x-axis). (C) The extent of rDNA and SMC4 co-localization at chromatids of metaphase chromosomes is presented in graphical format. The number of individuals chromatids examined is indicated at the top of each bar. A significant reduction in SMC4 co-localization at rDNA was observed on metaphase chromatids from cells expressing dnTRF2 for 36 hours. Scale bars in (A) are 15 micrometers.