PR inhibitory effects to cancer cell mobility are mediated by SDF-1 and IL-6.

<p>WPMY-1 cells expressing mock, PRA or PRB were transiently transfected with control siRNA or siRNA against PR for 24 hours. Cells were washed twice with PBS buffer and replenished with serum free DMEM medium for 48 hours. CM were collected and incubated with PC-3 (<b>A and C</b>) or C4-2B (<b>B and D</b>) cells for cell migration assays (<b>A and B</b>) and Matrigel invasion assays (<b>C and D</b>) as described in Material and Method section. (<b>E</b>) CM were collected from hCAFs in the presence of vehicle or 10 nM of P4 and then mixed with vehicle, 10 ng/ml of SDF-1 or 10 ng/ml IL-6 and incubated with PC-3 cells in Matrigel invasion assays. One-way ANOVA and paired student's t-test calculate the level of significance set at P<0.05 as * and P<0.001 as ***.