Combined anti-cancer effect of 2ME and CsA in pancreatic cancers in vitro.

<p><b>A.</b> MTT results showed the 2ME and CsA effect on cell proliferation. After treated by 2ME and/or CsA in BXPC3 cells, cell proliferation was initially similar in the interference and control groups (P>0.05). The proliferative ability in the 2ME and/or CsA treated groups significantly decreased at 48 h to 72 h compared to the control group (P<0.05, respectively: Figure. 4A); and the combination of 2ME and CsA decreased proliferative ability compared to 2ME or CsA alone (P<0.05, respectively). <b>B.</b> FACS results showed the 2ME and CsA effect on cell apoptosis. After treated by 2ME and/or CsA in BXPC3 cells, cell apoptosis was increased compared to the control group (P<0.05, respectively); and the combination of 2ME and CsA increased cell apoptosis compared to 2ME or CsA alone (P<0.05, respectively). <b>C.</b> The scratch results showed the 2ME and CsA effect on cell migaration. The scratch space at 0, 6, 12, and 24 h of BXPC3 cells was detected after treated by 2ME and/or CsA in BXPC3 cells: the widths in the 2ME and/or CsA treated groups were bigger than those in the control group at 12 and 24 h (P<0.05, respectively); and the combination of 2ME and CsA increased scratch space compared to 2ME or CsA alone (P<0.05, respectively). <b>D.</b> The transwell slide results showed the 2ME and CsA effect on cell invation. After treated by 2ME and/or CsA in BXPC3 cells, the transwell results showed the cell number through the membrane was significantly less than the control group (P<0.05); and the combination of 2ME and CsA decreased the cell number through the membrane compared to 2ME or CsA alone (P<0.05, respectively).