Over-expression of <i>OsGSTU4</i> in transgenic Arabidopsis plants.

<p>(A) Schematic representation of <i>35S:OsGSTU4</i> construct used for over-expression in Arabidopsis. The complete ORF of <i>OsGSTU4</i> was cloned in pBI121 vector carrying 35S promoter (B) Real-time PCR analysis of WT and transgenic Arabidopsis plants, showing the expression of <i>OsGSTU4</i> in five homozygous transgenic lines (19A, 21A, 25A, 26A and 28A) with no detectable expression in WT. The expression of <i>OsGSTU4</i> in homozygous transgenic lines are shown relative to 26A line exhibiting the lowest expression of the transgene. The expression of <i>PP2A</i> gene was used as internal control. Data are mean ± SE from three biological replicates.