Evaluation of tumor and EC apoptosis.

<p>Apoptosis and tumor angiogenesis was evaluated in tumor tissues by triple immunostaining with terminal transferase dUTP nick end labeling (TUNEL), CD31 and Topro-3. The tumor area was identified by H&E staining of adjacent sections. <b>(A–D)</b> Representative images of triple-immunostained tumors for TUNEL (red), CD31 (green) and Topro-3 (blue); Insets identified by dashed squares in A–D indicate higher magnification of the selected areas in solid squares. Arrowheads indicate TUNEL (+) cells (red); block arrows indicate CD31 (+) cells (green) and arrows indicate double TUNEL/CD31 (+) cells (red/green and yellow). <b>(E)</b> Quantification and graphic representation of only TUNEL (+) cells in all four treatment groups. <b>(F)</b> Quantification and graphic representation of double TUNEL/CD31 (+) cells in all four groups.