Effect of IPA-3 on cell growth and viability.

<p>(A) Cells were seeded at 2×10⁵ cells/ml and IPA-3 at different concentrations was added for 48 h. The number of viable cells (open symbols) and the necrotic cell fraction (closed symbols) were determined using Trypan blue exclusion test. CML-T1 and JURL-MK1 cells are shown as examples, the results for other cell lines are shown in Supporting Information, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0092560#pone.0092560.s001" target="_blank">Figure S1</a>. (B) Cells were seeded at 2×10⁵ cells/ml, incubated with 20 μM IPA-3 for 2 to 24 h and the metabolic activity was then measured using MTT assay as the percentage of the corresponding controls (untreated cells). White, light grey, dark grey and black bars correspond to 2, 10, 16 and 24 h incubation times, respectively. (C) Summary results from Trypan blue exclusion assay performed after 48 h incubation with 20 μM IPA-3. White bars: untreated controls, grey bars: IPA-3. At least 3 independent experiments were done for each cell line and for primary mononuclear cells from healthy donors (PBMC-HD), two experiments were performed using primary mononuclear cells from CML patients (PBMC-CML).