The effects of low temperature culture on the turnover of hKv1.5.

<p>(A) Chase analysis of the hKv1.5 expression, cultured at 37°C or 28°C for the indicated times after the treatment with cycloheximide, indicating the prolongation of the decay of the hkv1.5 immature protein cultured at low temperature. (B) Quantitative densitometry scans of the time-chase with cycloheximide treatment, cultured at 37°C or 28°C (*<i>P</i><0.05 vs 37°C). (C) The expression of hKv1.5 cultured at 37°C or 28°C after the treatment with MG132 (20 μM) for the indicated times. (D) Quantitative densitometry scans of the time-chase with MG132 treatment, cultured at 37°C or 28°C (**<i>P</i><0.01 vs time 0).