Colo699 microtissue protein expression pattern.

<p>IHC slices of Colo699 in mono- and co-cultures after five and ten days (Bar in A/C: 50 μm, bar in B/D: 100 μm, bar in Insert: 25 μm); Compared to monocultures Colo699 co-cultures formed much smaller spheroids. However, positive staining reactions for E-Cadherin and vimentin are comparable to the monocultures in the period of ten days. after ten days because no difference in the staining pattern between five and ten days was detected. Microtissues were completely positive for vimentin and Ki67, whereas no E-Cadherin and α-SMA expression was observed.