The effect of E-64 on viability of adult parasites and microfilariae.

<p>(A) Adult female worms (n = 10) of equal size were incubated in 20 ml maintenance at specified experimental conditions and viability was assessed by MTT assay after 8 h of exposure. Worms incubated in maintenance medium only served as control. (B) Microfilariae (mf) recovered from the uterus of gravid female of control and treated set. Adult female parasites were dissected longitudinally and the released mf was visualized under microscope at 40× (Motic B1 series). Data expressed is Mean ± SEM of at least three values (n = 3). ^***P<0.0001, ^**P<0.001, ^*P<0.05. Values with P<0.05 were considered significant.