The effects of neutralizing antibodies to LYVE-1 on LMW-HA-induced LECs tube formation.

<p>(<b>A</b>) SVEC4-10 cells were plated on matrigel in 96-well plates in complete medium contain LMW-HA (3.13 μg/ml), FGF-2 (20 ng/ml), CSA (3.13 μg/ml) or medium only. In some cases, cells were pre-treated with neutralizing anti-LYVE-1 antibodies or isotype IgG for 30min and then LMW-HA was added to reach a final concentration of 3.13 μg/ml and incubated for another 24 h. (<b>B</b>) Tube formation was quantitated by determining the total length of tubes. All experiments were repeated at least three times and show a representative example. Data points are fold increase over control. Magnification was×100. **, <i>p</i><0.01. ***, <i>p</i><0.001.