<p>(A) Ratios of beating cardiac foci corrected for total EBs plated in 8 independent experiments, and average ratio. Elimination of endogenous CS by ChABC led to a consistent, approximately 60% reduction in the number of beating cardiac foci. (*: p<0.05). (B) Time course of cardiac foci development in EB cultures. In 4 independent experiments, ChABC treatment lead to decreased numbers of cardiac foci even at d8 and d9, the earliest time points beating foci could be detected.</p
<p>(A) Real-time PCR result showing significant modulation of mRNA expression of Cyclins D1, D2, and...
<p><b>A,</b> Co-staining of BrdU and cardiac markers (a-actinin) to determine the effect of NCMs co-...
<p>(<b>A</b>) Current clamp recordings revealed differentiation of FACS-isolated cells into cardiac ...
<p>(A) Treatment of EB cultures with exogenous CS-E, but not C4S, from d0 to d4 significantly decrea...
<p>(A) Morphology of EB cultures on d4 and d12 of differentiation, in the presence or absence of ChA...
<p>(A) Western blot analysis of TGFbeta, BMP, MAPK and Wnt/beta-catenin signaling pathways in contro...
<p>X-gal stained embryoid bodies show early enrichment in cardiac-MHC-LacZ expressing areas (blue st...
<p>(A) Schematics for cardiac differentiation of P19 cells treated with 0.32 μM CsA, 1% DMSO, or CsA...
<p>(A) The CS-E-specific antibody GD3G7 detected widespread expression of CS-E moieties (no treatmen...
<p><b>A.</b> Flow cytometry analysis of RFP fluorescence in differentiating RFP6 Embryoid Bodies (EB...
<p>Endogenous CS and exogenous CS-E inhibit Wnt/beta-catenin signaling in EB cultures, leading to a ...
<p>Counterstaining for α-actinin and c-Kit in EBs exposed to 100 nM STS for 5 h and then cultured fo...
<p><b>A,</b> Time course quantification of spontaneous beating activity of differentiated cardiomyoc...
<p>EBs were formed by culturing P19 cells in suspension in non-adhesive Petri dishes for 48 or 96 h ...
(A) qPCR analysis of two individual Ccbe1 KD ESC clones (Clone 1 and Clone 2) compared to SH Control...
<p>(A) Real-time PCR result showing significant modulation of mRNA expression of Cyclins D1, D2, and...
<p><b>A,</b> Co-staining of BrdU and cardiac markers (a-actinin) to determine the effect of NCMs co-...
<p>(<b>A</b>) Current clamp recordings revealed differentiation of FACS-isolated cells into cardiac ...
<p>(A) Treatment of EB cultures with exogenous CS-E, but not C4S, from d0 to d4 significantly decrea...
<p>(A) Morphology of EB cultures on d4 and d12 of differentiation, in the presence or absence of ChA...
<p>(A) Western blot analysis of TGFbeta, BMP, MAPK and Wnt/beta-catenin signaling pathways in contro...
<p>X-gal stained embryoid bodies show early enrichment in cardiac-MHC-LacZ expressing areas (blue st...
<p>(A) Schematics for cardiac differentiation of P19 cells treated with 0.32 μM CsA, 1% DMSO, or CsA...
<p>(A) The CS-E-specific antibody GD3G7 detected widespread expression of CS-E moieties (no treatmen...
<p><b>A.</b> Flow cytometry analysis of RFP fluorescence in differentiating RFP6 Embryoid Bodies (EB...
<p>Endogenous CS and exogenous CS-E inhibit Wnt/beta-catenin signaling in EB cultures, leading to a ...
<p>Counterstaining for α-actinin and c-Kit in EBs exposed to 100 nM STS for 5 h and then cultured fo...
<p><b>A,</b> Time course quantification of spontaneous beating activity of differentiated cardiomyoc...
<p>EBs were formed by culturing P19 cells in suspension in non-adhesive Petri dishes for 48 or 96 h ...
(A) qPCR analysis of two individual Ccbe1 KD ESC clones (Clone 1 and Clone 2) compared to SH Control...
<p>(A) Real-time PCR result showing significant modulation of mRNA expression of Cyclins D1, D2, and...
<p><b>A,</b> Co-staining of BrdU and cardiac markers (a-actinin) to determine the effect of NCMs co-...
<p>(<b>A</b>) Current clamp recordings revealed differentiation of FACS-isolated cells into cardiac ...