<p>Note: E means reaction efficiencies and R<sup>2</sup> means Pearson’s coefficients of determination. Average Tm represents the mean melting temperature of the forward (Fw) and reverse (Rv) primers. Each sample contains 3 biological replicates.</p
<p><i>M</i>: expression stability average; SV: stability value; SD: standard deviation of C<sub>q</s...
<div><p>Quantitative real time RT-PCR has been described as the most sensitive method for the detect...
<p>(A) Expression of reference genes for 5 <i>V</i>. <i>parahaemolyticus</i> strains. (B) Expression...
a<p>: F, forward primer; R, reverse primer.</p>b<p>: Tm, Melting temperature.</p>c<p>: E, Efficiency...
<p><b>A</b>. Gene expression normalized with the best combination of reference genes selected by Nor...
Two methods, GeNorm and NormFinder, were used to evaluate four reference genes and to choose the bes...
<p>Expression levels of candidate reference genes in the different tissues by qRT-PCR (A); Average e...
<p><b>a.</b> Gene expression normalized with the most stable RGs selected by geNorm for the low temp...
<p>Average expression stability values (M) of reference genes were measured during stepwise exclusio...
Target and reference genes, primers sequences and melting temperatures for qPCR analysis.</p
<p>For the target genes, the real-time qRT-PCR derived relative expression levels (A, D and G for OP...
<p>Pair-wise variation (V<sub>n</sub>/V<sub>n+1</sub>, where n represents number of genes) was analy...
<p>A) Expression stability measurements (M) for the five reference genes analyzed. The x-axis from l...
<p>(A) Average expression stability values (M) following stepwise exclusion of the least stable refe...
<p>The average expression stability (M) of 16 candidate reference genes and the best combination of ...
<p><i>M</i>: expression stability average; SV: stability value; SD: standard deviation of C<sub>q</s...
<div><p>Quantitative real time RT-PCR has been described as the most sensitive method for the detect...
<p>(A) Expression of reference genes for 5 <i>V</i>. <i>parahaemolyticus</i> strains. (B) Expression...
a<p>: F, forward primer; R, reverse primer.</p>b<p>: Tm, Melting temperature.</p>c<p>: E, Efficiency...
<p><b>A</b>. Gene expression normalized with the best combination of reference genes selected by Nor...
Two methods, GeNorm and NormFinder, were used to evaluate four reference genes and to choose the bes...
<p>Expression levels of candidate reference genes in the different tissues by qRT-PCR (A); Average e...
<p><b>a.</b> Gene expression normalized with the most stable RGs selected by geNorm for the low temp...
<p>Average expression stability values (M) of reference genes were measured during stepwise exclusio...
Target and reference genes, primers sequences and melting temperatures for qPCR analysis.</p
<p>For the target genes, the real-time qRT-PCR derived relative expression levels (A, D and G for OP...
<p>Pair-wise variation (V<sub>n</sub>/V<sub>n+1</sub>, where n represents number of genes) was analy...
<p>A) Expression stability measurements (M) for the five reference genes analyzed. The x-axis from l...
<p>(A) Average expression stability values (M) following stepwise exclusion of the least stable refe...
<p>The average expression stability (M) of 16 candidate reference genes and the best combination of ...
<p><i>M</i>: expression stability average; SV: stability value; SD: standard deviation of C<sub>q</s...
<div><p>Quantitative real time RT-PCR has been described as the most sensitive method for the detect...
<p>(A) Expression of reference genes for 5 <i>V</i>. <i>parahaemolyticus</i> strains. (B) Expression...