The flow cytometric analysis of Kupffer cells/macrophages.

<p>Livers were harvested from mice 36-lipo or PBS, and liver MNCs obtained after collagenase digestion of livers were stained for F4/80, CD11b and CD68. The results of a forward scatter (FS)/side scatter (SS) analysis of the total MNCs are shown (left panels). The mean FS values are also shown (401±2.9 vs. 436±6.8, n = 5, p<005) (left panels). The F4/80/CD11b expression is also presented and the F4/80 positive populations are inside of square gate (middle panels). The CD11b/CD68 expression levels of the gated F4/80-positive cells are demonstrated (right panels). F4/80⁺ CD11b⁺ cells are shown by red dots and the F4/80⁺ CD68⁺ cells are shown by blue dots. **<i>p</i><0.01, *<i>p</i><0.05 vs. other groups. The data are representative of five mice in each group, with similar results for the five mice.