The serum MCP-1 levels after c-lipo treatment and the MCP-1 production from CD68⁺ Kupffer cells after CCl₄ injection with/without c-lipo pretreatment.

<p>(A) There was an increase in the serum MCP-1 levels in mice early after c-lipo injection. The mice were i.p. injected with c-lipo or PBS, the sera were obtained at the indicated time points, and the MCP-1 levels were measured (n = 3 in each group). (B) The serum MCP-1 levels did not increase after CCl₄ injection in mice pretreated with c-lipo. The mice pretreated with c-lipo or PBS were injected intraperitoneally with CCl₄ or oil, and sera were obtained at the indicated time points to measure the MCP-1 levels (n = 3 in each group).(C) The <i>ex vivo</i> production of MCP-1 from the liver CD68⁺ Kupffer cells from CCl₄-injected mice. One hour after the injection of CCl₄, the liver MNCs were harvested from the liver by collagenase treatment, and CD68⁺ cells were obtained by magnetic beads (MACS system). F4/80⁺ CD11b⁺ cells obtained by F4/80 magnetic beads from the liver MNCs from c-lipo treated mice. Both purified subsets were cultured <i>in vitro</i> for the indicated amounts of time. The data are the means ± SE from three independent experiments. **<i>p</i><0.01, *<i>p</i><0.05 vs. other groups.