K562 cell adhesion to RGD containing extracellular matrix proteins is restored in the presence of troglitazone and 9-<i>cis</i>-RA in a concentration and time dependent manner.

<p>(A) K562 cells cultured for 72 hrs in the presence of vehicle (<i>white bars</i>), 1 μM 9-<i>cis</i>-RA (<i>hatched bars),</i> 15 μM troglitazone (<i>gray bars)</i>, or 1 μM 9-<i>cis</i>-RA and 15 μM troglitazone (<i>black bars)</i> were added to wells coated with 5 μg/mL of fibronectin, FN-120, or vitronectin. (B) K562 cells cultured for 24, 48, or 72 hrs in the presence of vehicle (<i>white bars</i>), 1 μM 9-<i>cis</i>-RA (<i>hatched bars),</i> 15 μM troglitazone (<i>gray bars)</i>, or 1 μM 9-<i>cis</i>-RA and 15 μM troglitazone (<i>black bars)</i> were added to wells coated with 5 μg/mL of FN-120. (C) Microtiter wells were coated with various concentrations of FN-120. K562 cells cultured for 72 hrs in the presence of vehicle (▪), 1 μM <i>9-cis-</i>RA (•), 15 μM troglitazone (▴), or 1 μM <i>9-cis-</i>RAand 15 μM troglitazone (○), were added to wells (9×10⁴ cells/well) in Hepes-Tyrodes buffer containing 1 mM MnCl₂. Results are expressed as means ± SD, n = 6, LSD was used to test for differences among groups. Means followed by the same letter are not significantly different (P = 0.01).