<p>a, HEK-293 cells stably expressing HCA<sub>1</sub> were transfected with specific arrestin siRNA or a nonspecific control siRNA, 72 hrs after transfection, cells were harvested, and equal amounts of total cellular lysate were separated by 10% SDS-PAGE, transferred to a PVDF membrane, and incubated with anti-arrestin2/3 antibody. b, HEK-293 cells stably expressing HCA<sub>1</sub>-EGFP were transfected with specific arrestin siRNA or a non-specific control siRNA,72 hrs after transfection, cells were stimulated with 20 mM lactate for 60 min and examined with confocal microscopy as described under ‘Experimental Procedures.’ c, 72 hrs after transfection with specific arrestin siRNA or non-specific control siRNA, cells were stimulated with 20 ...
<p>Time course of Hu210-mediated ERK phosphorylation in <b>A</b>, N2A<sup>CB1R</sup>; <b>B</b>, N2A<...
<p>Western blot analysis of phospho-ERK1/2 (p-ERK1/2), total ERK1/2 (t-ERK1/2), phospho-p38MAPK (p- ...
<p><b>A, B, C:</b> Activation of PKC, cdk-2 and caspase-3 in permeabilized HeLa cells by H1. Y-axis:...
<p>a, Serum-starved CHO-HCA<sub>1</sub> cells were pretreated with DMSO or tyrphostin A9 (1 μM) for ...
<p><b>A.</b> LH86 cells were treated with different doses of ABT-263 as indicated for 1 h. Cells wer...
<p>The next day, cells were then stimulated with various concentrations of L-Lactate (a and b) or 3,...
<p>HA-tagged ERK2 was co-expressed with Flag-tagged WT arrestin-2 (<b>A,B,C</b>), or arrestin-3 (<b>...
<p>siRNA directed towards β-arrestin 1 and 2 were transfected into MEFs; 72 hours later the cells we...
<p>A. HCV replicon cells were transfected with siERK-1 (50 nM and 300 nM) or unrelated control siRNA...
<p>CHO-HCA<sub>1</sub> cells (a) or L6 cells (b) were cultured in serum-free DMEM/F12 or DMEM medium...
<p><b>(A)</b> Representative images of western blots for total and phosphorylated ERK1/2 in HEK293T ...
<p>(A) CHO cells stably expressing <i>wild-type</i> muscarinic M3 receptor (CHO-M3 cells) were trans...
<p>(<b>A</b>, <b>B</b> and <b>C</b>) Anoikis analysis is shown for (<b>A</b>) SMMC-7221 cells expres...
<p>For (A) and (B) the hepatoma cell lines Huh7 and Huh7.5 were cultured for 48 hours while primary ...
<p><b>A</b>. Active (phosphorylated at T183 and Y185 by MEK1) or inactive ERK2 (30 pmol) was pre-inc...
<p>Time course of Hu210-mediated ERK phosphorylation in <b>A</b>, N2A<sup>CB1R</sup>; <b>B</b>, N2A<...
<p>Western blot analysis of phospho-ERK1/2 (p-ERK1/2), total ERK1/2 (t-ERK1/2), phospho-p38MAPK (p- ...
<p><b>A, B, C:</b> Activation of PKC, cdk-2 and caspase-3 in permeabilized HeLa cells by H1. Y-axis:...
<p>a, Serum-starved CHO-HCA<sub>1</sub> cells were pretreated with DMSO or tyrphostin A9 (1 μM) for ...
<p><b>A.</b> LH86 cells were treated with different doses of ABT-263 as indicated for 1 h. Cells wer...
<p>The next day, cells were then stimulated with various concentrations of L-Lactate (a and b) or 3,...
<p>HA-tagged ERK2 was co-expressed with Flag-tagged WT arrestin-2 (<b>A,B,C</b>), or arrestin-3 (<b>...
<p>siRNA directed towards β-arrestin 1 and 2 were transfected into MEFs; 72 hours later the cells we...
<p>A. HCV replicon cells were transfected with siERK-1 (50 nM and 300 nM) or unrelated control siRNA...
<p>CHO-HCA<sub>1</sub> cells (a) or L6 cells (b) were cultured in serum-free DMEM/F12 or DMEM medium...
<p><b>(A)</b> Representative images of western blots for total and phosphorylated ERK1/2 in HEK293T ...
<p>(A) CHO cells stably expressing <i>wild-type</i> muscarinic M3 receptor (CHO-M3 cells) were trans...
<p>(<b>A</b>, <b>B</b> and <b>C</b>) Anoikis analysis is shown for (<b>A</b>) SMMC-7221 cells expres...
<p>For (A) and (B) the hepatoma cell lines Huh7 and Huh7.5 were cultured for 48 hours while primary ...
<p><b>A</b>. Active (phosphorylated at T183 and Y185 by MEK1) or inactive ERK2 (30 pmol) was pre-inc...
<p>Time course of Hu210-mediated ERK phosphorylation in <b>A</b>, N2A<sup>CB1R</sup>; <b>B</b>, N2A<...
<p>Western blot analysis of phospho-ERK1/2 (p-ERK1/2), total ERK1/2 (t-ERK1/2), phospho-p38MAPK (p- ...
<p><b>A, B, C:</b> Activation of PKC, cdk-2 and caspase-3 in permeabilized HeLa cells by H1. Y-axis:...