Expression profiling of conserved (A) and novel miRNAs (B) was performed to validate the predicted miRNAs.

<p>Quantitative PCR was performed using TaqMan chemistry. The relative abundance (Y-axis) was calculated using the ΔΔCt method. <i>B. juncea</i> seedlings were subjected for varied durations to either high temperature stress either at 35°C (BJH-1) and 42°C (BJH-2) or salinity stress at 150 mM NaCl (BJS-1) and 250 mM NaCl (BJS-2) or drought stress using 20% PEG (BJD-1) and 300 mM mannitol (BJD-2). The mean of three independent biological replicates is presented.