Representative <i>in vitro</i> screening results for Aβ -lowering and anti-oligomerization activity.

<p>(A) Measurement of Aβ_1–42 and Aβ_1–40 in conditioned medium from Tg2576 primary neurons treated with different concentration of testing compound (B) Dose dependent inhibition of testing compound on synthetic Aβ_1–42 peptides aggregation into HMW oligomeric Aβ forms. Aβ_1–42 (final concentration 10 μM) was incubated with various concentrations of compound at 37°C for 24 hours. Bands were visualized by western blot analysis probed with 6E10 antibody. MW: molecular weight, CTRL is Aβ_1–42 without incubation, Lane 1–8, 0, 10, 20, 40, 80,160, 320 and 640 μM of compound. (C and D) SDS-PAGE of Aβ_1–42 (C) and Aβ_1–40 (D) in the presence or absence of testing compound following PICUP. Aβ_1–42 or Aβ_1–40 was cross-linked in the presence or absence of testing compound and the bands were visualized using silver staining. Lanes 1: molecular weight, lane 2: Aβ_1–42 or Aβ_1–40 peptides without PICUP reaction; lane 3: Aβ_1–42 or Aβ_1–40 peptides following PICUP reaction; Lanes 4 and 5: PICUP of Aβ peptides in the presence of an equimolar concentration (lane 4) or 10x molar excess (lane 5) of testing compound.