5-aza-CdR inhibits cell proliferation and migration.

<p>A, Cell viability was determined after 72 h incubation by MTT assay. Results were normalized to that of untreated control and are means of 5 experiments ± SD. B, Matrigel invasion assay. a, photomicrographs of cells that have passed through Matrigel. Original magnification, ×200. b, quantification of invasion. The number of cells that migrated through pores onto the lower side of the filter was counted. We compared the number of cells that migrated with the number of untreated E0 cells that migrated. The Y axis represents fold change of invasiveness (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0093478#s2" target="_blank">Materials and Methods</a> for details).*, <i>P</i><0.05.