Administration of anti-IL-22 antibody is associated with altered B and T cell responses.

<p>Experimental design was the same as for <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0093279#pone-0093279-g002" target="_blank">figure 2</a>. <b>A.</b> Sera from mice receiving either anti-IL-22 antibody or isotype control were analyzed for anti-collagen IgG1, IgG2a and IgG2b antibodies by ELISA. <b>B, C & D:</b> Splenocytes from mice receiving anti-IL-22 or isotype control were stimulated with anti-CD3 (5 ug/ml for 3 days) or collagen (100 ug/ml for 7 days) and IL-17A, IFN-γ and IL-10 were measured in culture supernatants by ELISA. For some experiments IL-10 was measured by real-time PCR from splenocytes of mice receiving anti-IL-22 antibody or isotype control and expressed as fold change over GAPDH. Data is representative of 2 independent experiments (8 mice per group) with each dot representing an individual mouse. <b>E:</b> Single cell suspensions of the paws from mice receiving anti-IL22 or isotype control were stimulated with anti-CD3 (5 ug/ml for 3 days) and IL-17A was measured in culture supernatants by ELISA. Each dot represents an individual mouse.