Induction of IL-22 response during arthritis.

<p><b>A:</b> DBA mice were immunized with collagen and CFA. Splenocytes (5×10⁶/ml) from naïve mice, mice during initiation phase (2 weeks following immunization with collagen and CFA) or arthritic mice were stimulated with collagen (100 ug/ml) for 7 days or unstimulated. Supernatants were analyzed for IL-22 by ELISA. Data shown is representative of 3 independent experiments with 3 mice per experiment. <b>B:</b> Splenocytes (5×10⁶/ml) from arthritic mice were stimulated with varying concentrations of collagen (0, 10, 50 or 100 ug/ml) for 7 days and IL-22 was measured in supernatant by ELISA. Data shown is representative of 3 independent experiments with 3 mice per experiment. <b>C:</b> Splenocytes from arthritic mice were stimulated with PMA/ionomycin and Brefeldin A for 6 hours, stained intra-cellularly for IL-17, IFN-γ or IL-22 and analyzed by flow-cytometry. Data shown is gated on mononuclear cells based on forward and side scatter. Data is representative of 3 independent experiments with 3 mice per experiment.