Disruption of <i>ZFP521</i> gene.

<p>(A) Structure of the wild-type allele of the <i>ZFP521</i> gene, targeting vector, and recombinant allele. E, EcoRI restriction sites; closed box, location of probe used for Southern blotting (Probe A) and Northern blotting (Probe B); arrowheads a–c, PCR primers for genotyping the mice. (B) Southern blot analysis of offspring from <i>ZFP521^+/</i>^Δ mice. Genomic DNAs were digested with EcoRI, and then subjected to Southern blotting. The 7.4-kb band, recombinant allele; 6.3-kb band, wild-type allele. (C) Northern blot analysis of total mRNA. Total RNA was extracted from mouse brain, and subjected to northern blotting. Hybridization of blots with labeled cDNA probe for β-actin (middle panel), with 18S rRNA and 28S rRNA (bottom panel) used as controls for RNA loading. (D) PCR genotyping using mouse tail. Primers a and b were used for detection of the wild-type allele (412 bp), and a and c were used for the knockout allele (550 bp).