Over-expression of <i>GSDMB-1/-2</i> increases cell motility.

<p>(A) Analysis of cell motility in MCF7-C, MCF7-G1 and MCF7-G2 cells by barrier assay. Images were taken at five days post-seeding. Panels a, d and g show phase-contrast images. Panels b-c, e-f, h-i show F-actin staining by confocal immunofluorescence in the indicated cells. Cell nuclei were counterstained with DAPI (blue). Panels c, f and i show a magnification of the morphology of migrating cells at the invasive front for each cell line. Bars = 150 μm middle panels, 61.16 μm right panels. (B) Quantification of the migrated distance from the edge of the coverslip (in μm) in barrier assays. Bars represent the mean value ± s.d. relative to control cells (MCF7-C) by one-way ANOVA test **0.001<<i>p</i><0.005. N = 3 independent experiments. (C) Proliferation assay by BrdU incorporation in MCF7-C control cells and MCF7-G1 (#7,#9) and MCF7-G2 (#4, #5) transfectant clones. Bars represent the mean value ± s.d. by one-way ANOVA test; ns, non-significant. N = 3 independent experiments.