Prediction software analysis of novel missense mutations detected in the present study.

<p><b>Polyphen-2</b> - score 0 to1 shows low to high confidence for probability of protein damaging.</p><p><b>SIFT</b>- mutations considered as pathogenic showing score <0.05.</p><p><b>Align GVGD</b>- higher the score higher the probability of protein damaging nature of mutation</p><p><b>MUpro</b> - higher the negativity in SVM score higher the probability of decrease in protein structure stability.</p><p>SVM- Support Vector Machine (SVM score – negative score for novel missense mutations showed the pathogenic probability).