Impaired migration of IECs along the crypt-villus axis in the ileum of Shp2 CKO mice.

<p><b>A</b>: Frozen sections of the ileum from 3- to 4-week-old control or Shp2 CKO mice at 2 h after BrdU injection were immunostained with mAbs to BrdU (red) and to β-catenin (green). Representative images are shown in the left panel. The number of BrdU-positive cells per crypt was determined (right panel). Data are means ± SE for 54 (control) or 77 (Shp2 CKO) crypts from a total of three (control) or four (Shp2 CKO) mice per group. N.S., not significant (Student's <i>t</i> test). <b>B</b>: Frozen sections of the colon from 3- to 4-week-old control or Shp2 CKO mice at 2 h after BrdU injection were immunostained as in <b>A</b>. The number of BrdU-positive cells per crypt was determined. Quantitative data are means ± SE for 54 (control) or 47 (Shp2 CKO) crypts from a total of three mice per group. N.S., not significant (Wilcoxon rank-sum test). <b>C</b>: Frozen sections of the ileum from 3- to 4-week-old control or Shp2 CKO mice at 2 days after BrdU injection were immunostained as in <b>A</b>. The distance from the crypt base to the farthest migrated BrdU-positive cells was measured. Quantitative data are means ± SE for 40 (control) or 48 (Shp2 CKO) villi from a total of three mice per group. ***<i>P</i><0.0001 (Student's <i>t</i> test). All scale bars are 100 μm.