Characterization of MDS-L cells.

<p>(<b>A</b>) Basal expression levels of miR-125a and miR-125b in myeloid leukemia and MDS cell lines. miRNA levels are represented as the relative value to expression in K562 cells. Expression of miR-125a in MDS-L cells was 5–10 times higher than that of miR-125b (note that relative levels of miR-125a were calculated independently from those of miR-125b). Data represent mean ± SEM of n = 4 independent experiments. (<b>B</b>) Spontaneous differentiation of MDS-L cells after a 7-day culture in methylcellulose, expressed as the relative expression of differentiation markers, assessed by qPCR in MDS-L colonies collected after 7 days of incubation in methylcellulose. Data represent mean ± SEM of n = 3 independent experiments. Statistical significance: *P<0.05.