DSF-Cu inhibits murine MPM allograft growth.

<p>Female BALB/c mice bearing AB12 tumors were treated with either vehicle (Control) or DSF-Cu as described in methods. Animals were monitored every day for tumor volumes (growth) as well as any adverse effects. Tumor volumes and weights were measured as noted in methods. (A) Four tumors from control and two tumors from treated groups are shown. (B) Histogram showing tumor volumes of the control and DSF-Cu-treated groups. Points, mean tumor volume in each experimental group; Bars, SE. (C) Formalin-fixed tumor xenograft biopsies from panel A were paraffin embedded, processed, and subjected to immuno-staining as described in methods. Representative photomicrographs (400× magnification) in the first, top row show hematoxylin and eosin (H&E) staining of the tissue sections. Representative photomicrographs (400× magnification) are also shown to indicate for apoptosis (by TUNEL assay; second row), levels of p27Kip1 (third row), and CARP-1 (fourth row) proteins as noted in methods.