Differentiation of embryoid bodies (EBs) into male germ stem cell (GSC)-like cells.

<p>(A) Effects of treatment (EB medium; Non-treated, EB medium+BMP4, EB medium+RA or EB medium +BMP4/RA group) of EBs on the morphological features and induction of GSC-like cells after culture for 4 days. Scale bars: 50 µm. a; undifferentiated CHA hES 15, b: embryoid body from CHA hES 15, c; EB medium, d; EB medium+BMP4, e; EB medium+RA, f; EB medium +BMP4/RA group (B) Immunocytochemical staining using anti-VASA antibody on 4-day-old EBs (the left panel) and dissociated cells (the right panel). The yellow box indicates the magnified area. The red arrows indicate the signals for VASA fluorescence. <b>Note:</b> Scale bars are 20 µm.